97th DOG Annual Meeting 1999

K91

IN VITRO COMPARISON OF A SERUMFREE 31º CORNEAL STORAGE MEDIUM VERSUS 'CONVENTIONAL' 2% FCS LONG-TERM TISSUE CULTURE CORNEAL STORAGE

J. Stoiber, J. Ruckhofer, K. Weikinger, W. Muss, A. Lametschwandtner, G. Grabner

More than two thirds of all human corneas processed in the European region are stored using medium-term organ culture, with media containing FCS as mandatory growth factor. A storage medium that does not require components of bovine origin would further reduce the risk of transmission of infectious diseases such as BSE.

Methods: Five pairs of corneas that were unsuitable for grafting were used for this in vitro study. Each pair was stored at 31ºC for 1, 2, 3, 4 or 5 weeks. One cornea of each pair was cultivated in 'conventional' medium containing FCS, the other one was kept in the new serumfree medium (Insight Biomed Inc., USA). Media were changed weekly. After the initial preparation and at each change of medium an evaluation of cell viability, a measurement of corneal thickness and an endothelial cell count was performed. After deswelling using conventional medium + dextran (for corneas stored in conventional medium) or Optisol-GS (for serumfree cultivated corneas) scanning and transmission electron microscopy was performed.

Results: No substantial difference in corneal thickness, viability of the cells and endothelial cell count (in all corneas > 2600 cells/mm2) was found. Scanning electron microscopy showed a complete endothelial cell layer on all corneas.

Conclusion: The findings indicate a potential clinical applicability of the tested serumfree storage medium.

Landesaugenklinik, LKA Salzburg, Müllner Hauptstr. 48, A-5020 Salzburg


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