97th DOG Annual Meeting 1999
T-CELL SUBPOPULATIONS AND BASAL CELL CARCINOMA
OF THE EYELID
R. H. Stiemer, B. Wannke, J. M. Rohrbach, A. Mayer, H.-J. Thiel, M. Zierhut
Background: To investigate the role of T-cell subpopulations in the blood of patients with basal cell carcinoma (BCC) of the eyelid.
Methods: 30 consecutive patients were included in the study. Blood samples were taken before tumor excision, 1 week, 1 month and every 3 months after operation. Each blood sample was analysed with flow cytometry regarding the lymphocyte subpopulations: CD3+, CD3+/CD4+, CD3+/CD8+ T-lymphocytes, NK-cells (Natural killer cells), CD16+/CD56+/CD3+ T-lymphocytes, B-lymphocytes and the T-cell receptor (TCR) gamma/delta.
Results: The ranges of lymphocyte subpopulations were as follows: CD3+/CD4+ 18%-67% preop. and 19%-64% postop. (normal range 34%-63%), CD3+/CD 8+7%-36% preop. and 6-37% postop. (normal range 15%-42%). Before operation, 10/30 (33%) patients showed an elevated CD4+/CD8+ ratio (mean 3.2) which decreased to normal levels (0.8-2.8) in the following months after operation. Within the normal range were B-lymphocytes (3%-20%) and CD16+/CD56+/CD3+ T-lymphocytes (2%-10%). 12% of the patients showed an elevated level of NK-cells: 30%-35% (normal range 5%-25%). TCR gamma/delta was in normal range in all blood samples.
1. CD4+/CD8+ T-cells are abnormal in a third of the patients before surgery, but normalize months after excision.
2. TCR gamma/delta seem not to be very much involved in this process.
fortuene grant No. 299 (J.M. Rohrbach)
Dept. Ophthalmology, Schleichstr. 12-16, D - 72076 Tuebingen