97th DOG Annual Meeting 1999



F. Wilhelm, A. Kramer, G. Jendral, T. Bredehorn, G. Duncker

Donor eyes are primary contaminated by different microorganisms. The conjunctiva has a physiological bacterial milieu and additionally there can livepathogenic microorganisms. Because of the presence of that microorganisms results the risk of survival or multiplying during the storage time or transmission of infections to the recipients with the graft.

Method: On the eye banks of Greifswald and Kiel are corneas stored for up to 30 days in organ culture for five and seven years. A rigorous regime of asepsis and antisepsis during the procedures of obtaining and preparing the donor tissue, sterilisation and desinfection of the instruments and devices and additionally the use of antibiotics and antimycotics prescript in the standard operation procedures of both institutes. Continually performed microbiological examinations up to a long time after transplantation are necessary to check the efficiency of that procedures. The findings must be carefully documented it is necessary to increase the security for the patients and for the surgeon.

Results: In the whole time of existing the three eye banks no case of transmission of infection after transplanting an organ cultured graft was known. Hundreds of corneas were transplanted successfully in both clinics. Additionally numerous donor corneas were given 10 other clinics.

Conclusions: In Establishing a cornea bank like in the eye department of the university Halle a rigorous and efficient regime of decontamination and prophylaxis of transmission of infection has to be established. The organ culture itself is an microbiological culture and consequently an additional security in the prophylaxis of transmitting infections and securing the quality. The presented model can be an instruction for well established eye banks as well as for eye banks developing.

Klinik und Poliklinik für Augenheilkunde der Martin-Luther-Universität, Magdeburger Straße 8, D - 06097 Halle