97th DOG Annual Meeting 1999

P446

CONTAMINATION WITH PERFLUORODECALIN ON SURGICAL INSTRUMENTS

M. Winter1, C. Winter2, B. Wiechens1

Occasionally sub-, intra- or epiretinal PFCL-droplets are found after intraoperative use of perfluorocarbon liquids (PFCL). Possible origin of these droplets are surgical instruments contaminated with PFCL. Purpose of this study was to quantify the contamination of PFCL on surgical instruments after contact with these liquids. In addition methods for cleaning these instruments were investigated.

Material and methods: Several surgical instruments (illumination probe, vitrectome, hooks, forceps) were dipped in perfluorodecalin (PFD) for 30 seconds. Then the instruments were transferred to a solvent (perfluoromethylcyclohexane) of PFCL. In this solvent PFD was quantified by gaschromatography. In additional experiments the instruments contaminated with PFD were rinsed with physiologic NaCl-solution or cleaned with a moistened swab followed by determination of the residual PFD contamination.

Results: Contamination with up to 5µl PFD was detected on the surface of all instruments. After rinsing with physiologic NaCl-solution or cleaning with a moistened swab contamination with PFD was not detectable.

Conclusions: Rinsing with physiologic NaCl-solution or cleaning with a moistened swab are effective methods for cleaning the investigated instruments from adherent PFD. These methods can not completely prevent the occurrence of retinal PFD-droplets because the intraocular contamination of the instruments with PFD may lead to accumulations at the sclerotomy sites when the instruments are removed from the eye. A confluence and formation of epiretinal PFD-droplets are possible. Sub- or intraretinal PFD-droplets possibly also origin from manipulation on the retina with PFD contaminated surgical instruments.

1 Dept. of Ophthalmology, University Kiel, Hegewischstr. 2, D-24105 Kiel
2 Pharamceutical Institute, University Kiel

Project in cooperation with the "Arbeitsgemeinschaft für Glaskörperersatz"


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