97th DOG Annual Meeting 1999

K43

ACCURACY OF AUTOMATED MORPHOMETRY OF CORNEAL ENDOTHELIUM USING THE TOMEY EM-1000 SPECULAR MICROSCOPE

H. Landenberger, C. Erb, T.-M. Wohlrab, H.-J. Thiel

Purpose: To evaluate the clinical use of a computer assisted image analyzing of human cornea endothelial specular microscopy

Methods: 243 eyes of 61 male and 69 female persons, 12 to 91 (58,8 +/-20,8) years old with no known alteration of corneal endothelium were examined using a TOMEY specular microscope EM-1000. The digitized images were analyzed with the inbuilt algorithm on an IBM compatible personal computer. Cell density was automatically calculated by seperating cell bodies through enhancing cell borders.

Results: The obtained values for cell density are different from those previously described. The higher density values of the juvenile corneal endothelium (about 3500 mm-2) were never seen, the age dependent decrease of density was smaller in our examinations. Mean value of cell density in our investigations was 2268,6 +/- 169,4 mm-2 and we found no statistically significiant difference between female and male persons.

Conclusion: An elegant and easy-to-use way for documenting of corneal endothelium morphology stands in contrast to the poor correlation between our investigations and the previously described normal values of corneal endothelium cell density and its specific age related decrease. To improve clinical usability further research into combining image analyzing algorithms is required.

Universitäts-Augenklinik, Abt. 1, Schleichstr. 12-16, D-72072 Tübingen


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