97th DOG Annual Meeting 1999
CALCIUM- AND MAGNESIUM FREE INFUSION FLUID FOR THE ASSISTANCE OF MACULAR TRANSLOCATION SURGERY
F. Faude1, A. Reichenbach2, S. Wendt2, P. Wiedemann1
Introduction: For macular translocation surgery the healthy retina has to be detached locally or completely. Because of the strong adhesion between the neurosensory retina and the retinal pigment epithelium it is difficult to detach the retina. A minimized calcium and magnesium reduces the adhesive force between RPE and the neurosensory retina. We studied the influence of a calcium- and magnesium free infusion fluid on the RPE and the photoreceptor outer segments.
Methods: Pigmented rabbits of both sexes were used. A pars plana vitrectomy was performed. With a thin glass micropipetette attached to a glass syringe a circumscript retinal detachment was created. In the first experience a balanced salt solution was used for the vitrectomy and the creating of the subretinal bleb. In a second experience the same procedure was performed by using a calcium- and magnesium free infusion fluid. The detached retina was examined in a microscop analyser.
Results: Using a Ca++- and Mg++ free infusion fluid facilitates and accelerates the retinal detachment. We observed less RPE adherence to the neural retina and a less photoreceptor outer segment swelling by using the Ca++ - and Mg++ free infusion fluid.
Conclusion: The Ca++- and Mg++ free infusion fluid facilitates the retinal detachment in macular translocation surgery and takes care of the RPE and the photoreceptor outer segments.
1University Eye Hospital, Leipzig,
2Paul-Flechsig-Institute for Brain Research, Leipzig