97th DOG Annual Meeting 1999
USE OF A MICROINJECTION PUMP FOR TRANSPLANTATION OF RETINAL PIGMENT EPITHEL IN THE SUBRETINAL SPACE OF RCS RATS
J. Weichel1, K. Engelmann1, M. Valtink1, H. Schäfer2, G. Richard1
Purpose: The succsess of transplantation of retinal pigment epithelial cells (RPE) in animal models was spoiled by insufficient cell concentration and "patchy" adhaesion of the donor cells in the recipient eye. For optimizing the surgical technique, the influence of different types of canules and injection systems on donor cell and recipient tissue integrity was examined.
Method: After passaging adult human RPE cells (cultured RPE cells of a RPE cell bank) through three different types of canules which were attached to either an insuline syringe or an oil-hydraulic micro-injection pump (Celltram Oil Pump), the examination of cell integrity as a function of a possible damage of the donor cells was carried out by a trypan blue exclusion test. Furthermore, adult human RPE cells were transplanted into the subretinal space of 10 RCS rats using an insuline syringe, an air-hydraulic pump (Celltram Air Pump) and an oil-hydraulic pump. After 24 hours the animals were killed and the eye sections were examined histologically for traumatic damages.
Results: Cell integrity rates (percentage of non-destroyed cells on total cell number) of 83-95% could be recorded when the insulin syringe combined with the three types of canules was employed. The integrity rates were 91-97% when the micro-injection pump combined with the three different canules was employed. Histological examination of the rat eyes (n=10) showed strong to medium degreed traumatic damages in 4(10) eyes for the insuline syringe, in 2(10) eyes for the Celltram Air Pump and in none for the Celltram Oil Pump.
Conclusion: It could be demonstrated that the use of the Celltram Oil Pump for cell transplantation minimizes traumatic effects on the donor cells and the recipient tissue. Apart from that it turned out that the canule-type does not influence the donor cell integrity.
1 University Eye Clinic, Martinistrasse 52, D-20246 Hamburg, Germany.
2 University Hamburg, Department of Pathology, Martinistr. 52 D-20246 Hamburg,